Czech Mycology 60(2): 231–242             Article published: 27th February, 2009 doi: 10.33585/cmy.60207

GRYNDLER M., BOROVIČKA J., GRYNDLEROVÁ H., GRYNDLER E.

Fructification of Langermannia gigantea in artificially inoculated field soil.

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Abstract

Cultures of Langermannia gigantea, a fungus potentially important for human alimentation and biotechnology purposes, were isolated from wild-growing specimens and a spawn was produced using autoclaved soil as a cultivation substrate. The spawn of two isolates was placed into the soil in two field experimental plots. Fructification was first observed after 4 years in only one plot, where 3 normal fruitbodies were produced. Analysis of the rDNA cassette of the 3 fruitbodies revealed that they were identical and bore the attributes of both introduced isolates. The fungus is able to persist in the inoculated soil for years and is dispersed over the soil volume. This is the first report of successful fructification of L. gigantea introduced at a locality where this species had never been observed before.


Keywords:     Lycoperdaceae, Langermannia gigantea, Calvatia gigantea, spawn, cultivation, rDNA

Full citation:

Gryndler M., Borovička J., Gryndlerová H., Gryndler E. (2008): Fructification of Langermannia gigantea in artificially inoculated field soil. – Czech Mycology 60(2): 231–242. copy to clipboard


doi: 10.33585/cmy.60207

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