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OSTRÝ V., RUPRICH J., KOŽÍŠEK J. (1998): Determination of toxigenic Fusarium spp. in the domestic wheat - using the ICFM methodological recommendation. [Fusarium spp., wheat, isolation, identification, mycotoxins, food mycology] Czech Mycology 50(4): 313-323 (published: 12th July, 1998)
abstract
Fifty one food wheat samples from three production regions in the Czech Republic have been mycologically examined in this study. Fusarium species were isolated by them ethod of grain rinse with sterile 0.1 % pepton in water and by the method of direct plating of grains after their surface sterilization. Czapek Dox Iprodione Dichlorane Agar (CZID) was used for cultivation. The methodological procedures used issued from there commendation of the International Commission of Food Mycology (ICFM). The identification of the isolated strains has been done accordingto Nelson et al. (1983) and by comparingwith collection strains of the genus Fusarium (Czech Collection of Microorganisms in Brno - CCM). Standardization of the above-mentioned mycological methods in food mycology is necessary for making collaborative studies and also for possibilities of comparison of results obtained in different time. Fusarium spp. isolated from food wheat samples of showed the greatest frequency in the following order: Fusarium graminearum, F. avenaceum, F. sporotrichioides, F. reticulatum and F. solani. The methodological procedure is recommended for determination of toxigenic Fusariumspp. in wheat and other cereals after a harvest and for comparison of results obtained both between individual production regions and in individual years.
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