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Search for "krupodorova t.a": 0 articles found.
Probable match (100%) "KRUPODOROVA": 2 articles found.
KRUPODOROVA T., BARSHTEYN V., KIZITSKA T., RATUSHNYAK V., BLUME Y. (2023): Antagonistic activity of selected macromycetes against two harmful micromycetes. [ascomycetes, Aspergillus niger, basidiomycetes, dual-culture, Penicillium polonicum.] Czech Mycology 75(1): 85-100 (published: 23rd June, 2023)
abstract
Competition between 31 macromycete species and two harmful micromycetes Aspergillus niger and Penicillium polonicum was evaluated using dual-culture plate assay. All investigated fungi, except for Inonotus obliquus and Lepista luscina, possessed different levels of antagonistic activity against the tested micromycetes. Hypsizygus marmoreus and Lyophyllum shimeji were inactive against A. niger. Morchella esculenta and Oxyporus obducens were passive in the case of co-growth with P. polonicum. The study of fungal interactions showed variability in types of reactions and level of their visualisation. Co-cultivation of the studied species resulted in the following reactions: deadlock after mycelial contact and at a distance, partial or complete replacement after initial deadlock on contact. In general, the studied macromycetes showed moderate activity against the two micromycetes, as 11 species showed active and 7 species moderate antagonism. Results revealed higher antagonistic activity of macromycetes against P. polonicum than in A. niger co-cultures. Wood-decaying species Ganoderma lucidum and Trametes versicolor were the most active fungi against the two tested micromycetes with a maximal antagonism index. These findings provide valuable insights which can be further explored by means of in vivo assays to find a suitable agent for the biocontrol of diseases or spoilage caused by A. niger and P. polonicum.
KRUPODOROVA T.A., BARSHTEYN V.YU., KIZITSKA T.O., POKAS E.V. (2019): Effect of cultivation conditions on mycelial growth and antibacterial activity of Lentinula edodes and Fomitopsis betulina. [xylotrophic basidiomycetes, shiitake, Piptoporus, fungal growth conditions, fungal antibiotic activity.] Czech Mycology 71(2): 167-186 (published: 13th December, 2019)
abstract
This research dealt with the effect of cultivation conditions on mycelial growth and antibacterial activity of Lentinula edodes and Fomitopsis betulina in liquid static culture. The antibacterial activity of mycelium and culture liquid were assayed against the bacteria Bacillus subtilis, Staphylococcus aureus and Escherichia coli using the disk diffusion method. The optimum incubation period of mycelial growth for both species and their antibacterial activity was 14 days. The biomass of the studied species was the largest at temperatures of 26–28 °C. The highest mycelium production was recorded at pH 3.5–4.0, whereas the strongest antibacterial activity was observed at pH 5.5. Of the carbon sources tested, the highest mycelium productivity of L. edodes was recorded for glucose, followed by cellulose. Cellulose was found to be the optimal carbon source for the highest antibacterial activity of L. edodes. Cellulose was also the optimal carbon source for mycelial growth in F. betulina, but the highest antibacterial activity was achieved with added galactose. For both fungal species, asparagine was found to be the nitrogen source producing a maximum biomass, whereas the highest antibacterial activity of L. edodes and F. betulina was noticed after adding ammonium nitrate and peptone, respectively. The antibacterial potential of culture liquids appeared to be higher in the studied species when compared to their mycelium. Establishing optimum cultivation conditions will allow for obtaining maximum productivity of mycelium and culture liquids with the highest antibacterial activity.
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