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KRUPODOROVA T.A., BARSHTEYN V.YU., KIZITSKA T.O., POKAS E.V. (2019): Effect of cultivation conditions on mycelial growth and antibacterial activity of Lentinula edodes and Fomitopsis betulina. [xylotrophic basidiomycetes, shiitake, Piptoporus, fungal growth conditions, fungal antibiotic activity.] Czech Mycology 71(2): 167-186 (published: 13th December, 2019)
abstract
This research dealt with the effect of cultivation conditions on mycelial growth and antibacterial activity of Lentinula edodes and Fomitopsis betulina in liquid static culture. The antibacterial activity of mycelium and culture liquid were assayed against the bacteria Bacillus subtilis, Staphylococcus aureus and Escherichia coli using the disk diffusion method. The optimum incubation period of mycelial growth for both species and their antibacterial activity was 14 days. The biomass of the studied species was the largest at temperatures of 26–28 °C. The highest mycelium production was recorded at pH 3.5–4.0, whereas the strongest antibacterial activity was observed at pH 5.5. Of the carbon sources tested, the highest mycelium productivity of L. edodes was recorded for glucose, followed by cellulose. Cellulose was found to be the optimal carbon source for the highest antibacterial activity of L. edodes. Cellulose was also the optimal carbon source for mycelial growth in F. betulina, but the highest antibacterial activity was achieved with added galactose. For both fungal species, asparagine was found to be the nitrogen source producing a maximum biomass, whereas the highest antibacterial activity of L. edodes and F. betulina was noticed after adding ammonium nitrate and peptone, respectively. The antibacterial potential of culture liquids appeared to be higher in the studied species when compared to their mycelium. Establishing optimum cultivation conditions will allow for obtaining maximum productivity of mycelium and culture liquids with the highest antibacterial activity.
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